Genetics of Parkinson's Disease: Lysosomal Dysfunction Antibody Sampler Kit (HAK21127)

Western blot analysis of VPS35 on different lysates with Rabbit anti-VPS35 antibody (HA723858) at 1/5,000 dilution.

Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: U-937 cell lysate (20 µg/Lane)
Lane 4: COS-1 cell lysate (20 µg/Lane)
Lane 5: Neuro-2a cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C2C12 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: NR8383 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse brain tissue lysate (40 µg/Lane)
Lane 12: Mouse colon tissue lysate (40 µg/Lane)
Lane 13: Rat brain tissue lysate (40 µg/Lane)
Lane 14: Rat colon tissue lysate (40 µg/Lane)

Predicted band size: 92 kDa
Observed band size: 80 kDa

Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723858) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.