XBP1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-23] (HA751891)
Catalog# HA751891
Rabbit Monoclonal to XBP1
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of XBP1 on different lysates with Rabbit anti-XBP1 antibody (HA751891) at 1/5,000 dilution.
Lane 1: Huh7 (Human liver cancer cells) cell lysate
Lane 2: Huh7 (Human liver cancer cells) treated with 300nM Thapsigargin for 18 hours cell lysate
Lane 3: C6 (Rat glioma cells) cell lysate
Lane 4: C6 (Rat glioma cells) treated with 300nM Thapsigargin for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 24 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751891, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 29, 40 kDa
Observed band size: 29, 56 kDa
Western blot analysis of XBP1 on different lysates with Rabbit anti-XBP1 antibody (HA751891) at 1/5,000 dilution.
Lane 1: Huh7 (Human liver cancer cells) cell lysate
Lane 2: Huh7 (Human liver cancer cells) treated with 300nM Thapsigargin for 18 hours cell lysate
Lane 3: C6 (Rat glioma cells) cell lysate
Lane 4: C6 (Rat glioma cells) treated with 300nM Thapsigargin for 18 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 24 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751891, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 29, 40 kDa
Observed band size: 29, 56 kDa