Wnt3a Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-96] (HA751737)
Catalog# HA751737
Rabbit Monoclonal to Wnt3a
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Wnt3a on HCT 116 cell lysates with Rabbit anti-Wnt3a antibody (HA751737) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751737) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa
Exposure time: 1 minute; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751737) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.