VEGFR3 / FLT4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH20-96] (HA751778)
Catalog# HA751778
Rabbit Monoclonal to VEGFR3 / FLT4
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of VEGFR3 / FLT4 on different lysates with Rabbit anti-VEGFR3 / FLT4 antibody (HA751778) at 1/5,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: A549 treated with 10ng/mL TNF alpha and 10nM TPA for 24 hours cell lysate (20 µg/Lane)
Predicted band size: 153 kDa
Observed band size: 180 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751778) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: A549 treated with 10ng/mL TNF alpha and 10nM TPA for 24 hours cell lysate (20 µg/Lane)
Predicted band size: 153 kDa
Observed band size: 180 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751778) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.