ULK3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH01-09] (HA750685)
Catalog# HA750685
Rabbit Monoclonal to ULK3
Tested for
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WB
-
IHC-P
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IF-Cell
-
FC
-
IF-Tissue
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (HA750685) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750685) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750685) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.