ULK1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JA58-36] (HA750418)

UNIPROT #

O75385

PROTEIN NAME

ULK1

SYNONYMS

ATG 1 antibody;ATG1 antibody;ATG1 autophagy related 1 homolog antibody;ATG1A antibody;Autophagy related protein 1 homolog antibody;Autophagy-related protein 1 homolog antibody;FLJ38455 antibody;FLJ46475 antibody;hATG1 antibody;KIAA0722 antibody;Serine/threonine protein kinase ULK1 antibody;Serine/threonine protein kinase Unc51.1 antibody;Serine/threonine-protein kinase ULK1 antibody;ULK 1 antibody;ULK1 antibody;ULK1_HUMAN antibody;Unc 51 (C. elegans) like kinase 1 antibody;UNC 51 antibody;Unc 51 like kinase 1 antibody;Unc-51 like kinase 1 (C. elegans) antibody;Unc-51-like kinase 1 antibody;UNC51 antibody;UNC51, C. elegans, homolog of antibody;Unc51.1 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.

TISSUE SPECIFICITY

Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated. Phosphorylated under nutrient-rich conditions; dephosphorylated during starvation or following treatment with rapamycin. Under nutrient sufficiency, phosphorylated by MTOR/mTOR, disrupting the interaction with AMPK and preventing activation of ULK1 (By similarity). In response to nutrient limitation, phosphorylated and activated by AMPK, leading to activate autophagy.; Acetylated by KAT5/TIP60 under autophagy induction, promoting protein kinase activity.

SUBCELLULAR LOCATION

Cytosol, Preautophagosomal structure.

FUNCTION

Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.