TRPA1 Recombinant Rabbit Monoclonal Antibody [PSH23-56] (HA724319)
Catalog# HA724319
Rabbit Monoclonal to TRPA1
Tested for
-
WB
Reacts with
-
Human
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TRPA1 on different lysates with Rabbit anti-TRPA1 antibody (HA724319) at 1/5,000 dilution.
Lane 1: U-2 OS (Human osteosarcoma cells) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 4: IMR-32 (Human neuroblastoma cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724319, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 127 kDa
Observed band size: 150 kDa
Lane 1: U-2 OS (Human osteosarcoma cells) cell lysate
Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 3: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 4: IMR-32 (Human neuroblastoma cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724319, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 127 kDa
Observed band size: 150 kDa