Transmembrane protein 200A Recombinant Mouse Monoclonal Antibody - BSA and Azide free [4-B3-R] (HA610071)
Catalog# HA610071
Mouse Monoclonal to Transmembrane protein 200A
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Transmembrane protein 200A on different lysates with Mouse anti-Transmembrane protein 200A antibody (HA610071) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: PC-3M cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 47 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610071) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: PC-3M cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 47 kDa
Exposure time: 45 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610071) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.