TMEM50A Rabbit Polyclonal Antibody (HA500556)
Catalog# HA500556
Rabbit Polyclonal to TMEM50A
Tested for
-
WB
Reacts with
-
Mouse
Western blot analysis of TMEM50A on different lysates with Rabbit anti-TMEM50A antibody (HA500556) at 1/1,000 dilution.
Lane 1: Mouse colon tissue lysate
Lane 2: Mouse lung tissue lysate (no heat)
Lane 3: Mouse kidney tissue lysate (no heat)
Lane 4: Mouse spleen tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500556) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse colon tissue lysate
Lane 2: Mouse lung tissue lysate (no heat)
Lane 3: Mouse kidney tissue lysate (no heat)
Lane 4: Mouse spleen tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 30 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 35 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500556) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.