TMED9 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A9A7-R] (HA610040)
Catalog# HA610040
Mouse Monoclonal to TMED9
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of TMED9 on different lysates with Mouse anti-TMED9 antibody (HA610040) at 1/1,000 dilution.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: LO2 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: A431 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: Raji cell lysate
Lane 9: PC-12 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: Human lung tissue lysate
Lane 12: Mouse lung tissue lysate
Lane 13: Mouse liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 10 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: LO2 cell lysate
Lane 4: THP-1 cell lysate
Lane 5: A431 cell lysate
Lane 6: A549 cell lysate
Lane 7: MCF-7 cell lysate
Lane 8: Raji cell lysate
Lane 9: PC-12 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: Human lung tissue lysate
Lane 12: Mouse lung tissue lysate
Lane 13: Mouse liver tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 10 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610040) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.