TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH21-35] (HA724218)
Catalog# HA724218
Rabbit Monoclonal to TIMP1
Tested for
-
WB
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (HA724218) at 1/5,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: Neuro-2a cell lysate (negative)
Lane 3: MEF cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724218) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (HA724218) at 1/5,000 dilution.
Lane 1: C2C12 cell lysate
Lane 2: Neuro-2a cell lysate (negative)
Lane 3: MEF cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724218) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.