Thrombomodulin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-10] (HA751888)
Catalog# HA751888
Rabbit Monoclonal to Thrombomodulin
Tested for
-
WB
-
IF-Cell
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IHC-P
-
FC
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Thrombomodulin on different lysates with Rabbit anti-Thrombomodulin antibody (HA751888) at 1/20,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 2: 293T (Human embryonic kidney cells) cell lysate
Lane 3: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
293T is negative control (PMID: 40414460).
Negative expression of Thrombomodulin protein in Jurkat cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751888, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 60 kDa
Observed band size: 75-100 kDa
Western blot analysis of Thrombomodulin on different lysates with Rabbit anti-Thrombomodulin antibody (HA751888) at 1/20,000 dilution.
Lane 1: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 2: 293T (Human embryonic kidney cells) cell lysate
Lane 3: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
293T is negative control (PMID: 40414460).
Negative expression of Thrombomodulin protein in Jurkat cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751888, 1/20,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 60 kDa
Observed band size: 75-100 kDa