Thioredoxin Recombinant Rabbit Monoclonal Antibody [PSH23-52] (HA724383)
Catalog# HA724383
Rabbit Monoclonal to Thioredoxin
Tested for
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WB
-
IP
Reacts with
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Human
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Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Thioredoxin on different lysates with Rabbit anti-Thioredoxin antibody (HA724383) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: Hep G2 (Human liver cancer cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: A549 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 9 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724383, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 12 kDa
Observed band size: 12 kDa
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: Hep G2 (Human liver cancer cells) cell lysate
Lane 3: MCF7 (Human breast cancer cells) cell lysate
Lane 4: A549 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 9 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724383, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 12 kDa
Observed band size: 12 kDa