Strep-Tag II Recombinant Rabbit Monoclonal Antibody [PSH05-14] (HA722230)
Catalog# HA722230
Rabbit Monoclonal to Strep-Tag II
Tested for
-
WB
-
IF-Cell
-
IP
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Strep-Tag II on different lysates with Rabbit anti-Strep-Tag II antibody (HA722230) at 1/2,000 dilution.
Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate
Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722230) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T transfected with Strep-Tag II-tagged Histone H3.1 (C-terminal) cell lysate
Lane 2: 293T transfected with Strep-Tag II-tagged ACAT2 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722230) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.