Steroid sulfatase Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH05-74] (HA751024)
Catalog# HA751024
Rabbit Monoclonal to Steroid sulfatase
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Steroid sulfatase on different lysates with Rabbit anti-Steroid sulfatase antibody (HA751024) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: LNCaP cell lysate (negative)
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: Huh7 cell lysate
Lane 6: T-47D cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751024) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Steroid sulfatase on different lysates with Rabbit anti-Steroid sulfatase antibody (HA751024) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: LNCaP cell lysate (negative)
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: Huh7 cell lysate
Lane 6: T-47D cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751024) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.