SLC7A5 / LAT1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-28] (HA751282)
Catalog# HA751282
Rabbit Monoclonal to SLC7A5 / LAT1
Tested for
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WB
-
IF-Cell
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IHC-P
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of SLC7A5 / LAT1 on different lysates with Rabbit anti-SLC7A5 / LAT1 antibody (HA751282) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (negative) (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Lane 5: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 37 kDa
Exposure time: Lane 1-2: 1 minute; Lane 3-5: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751282) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SLC7A5 / LAT1 on different lysates with Rabbit anti-SLC7A5 / LAT1 antibody (HA751282) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: LNCaP cell lysate (negative) (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: Mouse testis tissue lysate (40 µg/Lane)
Lane 5: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 37 kDa
Exposure time: Lane 1-2: 1 minute; Lane 3-5: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751282) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.