SCXA Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH02-25] (HA750793)
Catalog# HA750793
Rabbit Monoclonal to SCXA
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody (HA750793) at 1/1,000 dilution.
Lane 1: HEK-293-NT cell lysate
Lane 2: HEK-293-OE-SCXA cell lysate
Lane 3: HeLa-NT cell lysate
Lane 4: HeLa-OE-SCXA cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 27 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750793) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SCXA on different lysates with Rabbit anti-SCXA antibody (HA750793) at 1/1,000 dilution.
Lane 1: HEK-293-NT cell lysate
Lane 2: HEK-293-OE-SCXA cell lysate
Lane 3: HeLa-NT cell lysate
Lane 4: HeLa-OE-SCXA cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 22 kDa
Observed band size: 27 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750793) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.