SAV1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH04-45] (HA750954)
Catalog# HA750954
Rabbit Monoclonal to SAV1
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (HA750954) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: SW480 cell lysate (20 µg/Lane)
Lane 7: BxPC-3 cell lysate (20 µg/Lane)
Lane 8: 786-0 cell lysate (negative) (20 µg/Lane)
Lane 9: A549 cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: PC-12 cell lysate (20 µg/Lane)
Lane 12: Mouse testis tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes;
ECL: Lane 1-13 (left): K1801; Lane 1-13 (right): K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750954) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of SAV1 on different lysates with Rabbit anti-SAV1 antibody (HA750954) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: 293T cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: MDA-MB-231 cell lysate (20 µg/Lane)
Lane 6: SW480 cell lysate (20 µg/Lane)
Lane 7: BxPC-3 cell lysate (20 µg/Lane)
Lane 8: 786-0 cell lysate (negative) (20 µg/Lane)
Lane 9: A549 cell lysate (20 µg/Lane)
Lane 10: NIH/3T3 cell lysate (20 µg/Lane)
Lane 11: PC-12 cell lysate (20 µg/Lane)
Lane 12: Mouse testis tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes;
ECL: Lane 1-13 (left): K1801; Lane 1-13 (right): K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750954) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.