ROR gamma T Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH04-38] (HA750947)
Catalog# HA750947
Rabbit Monoclonal to ROR gamma T
Tested for
-
WB
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IHC-P
Reacts with
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Human
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of ROR gamma T on different lysates with Rabbit anti-ROR gamma T antibody (HA750947) at 1/2,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: Rat thymus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750947) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate
Lane 2: Rat thymus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750947) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.