Recombinant Mouse IgG1 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [PSH04-91] (HA610204)
Catalog# HA610204
Mouse Monoclonal to Recombinant Mouse IgG1
Tested for
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WB
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FC
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IP
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ChIP
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
PCNA was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601172 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601172 at 1/10,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (input)
Lane 2: HA601172 IP in HeLa cell lysate
Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 59 seconds; ECL: K1801
Lane 1: HeLa cell lysate (input)
Lane 2: HA601172 IP in HeLa cell lysate
Lane 3: Recombinant Mouse IgG1 (HA610204) instead of HA601172 in HeLa cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 1 minute 59 seconds; ECL: K1801