Rb Antibody Sampler Kit (HAK21105)
Catalog# HAK21105
Reacts with
-
Human
Western blot analysis of Phospho-Rb (S795) on different lysates with Rabbit anti-Phospho-Rb (S795) antibody (HA722764) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate (20 µg/Lane)
Lane 3: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722764) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate (20 µg/Lane)
Lane 3: HeLa treated with 100ng/mL Nocodazole for 18 hours cell lysate, then the membrane treated with λpp for 1 hour (20 µg/Lane)
Predicted band size: 106 kDa
Observed band size: 106 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722764) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.