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Rat IL-4 enzyme-linked immunoassay kit (ER0003)

Price: Regular price $499.00

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Catalog# ER0003

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 17.29 pg/ml (50 μl)

Reacts with

  • Rat

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.1050.1040.1045
2.740.10860.10360.10610.0016
8.230.110.1080.1090.0045
24.690.13820.14330.14080.0767
74.070.28120.29530.28830.2242
222.220.64220.65430.64830.5842
666.671.54321.51531.52931.4652
20003.08813.19323.14073.0766

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)33.4172.8557.432.8175.6561.2
Standard Deviation1.88.126.82.312.134.8
Coefficient of Variation(%)5.44.74.876.96.2

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.00

Sensitivity

17.29 pg/ml (50 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of rat IL-4 into health rat serum sample. The un-spiked serum was used as blank in these experiments. The recovery ranged from 93% to 115% with an overall mean recovery of 105%.

Specifications

Product Type

ELISA Kit

Product Name

Rat IL-4 enzyme-linked immunoassay kit (ER0003)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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