Rat IL-10 enzyme-linked immunoassay kit (ER0005)
Catalog# ER0005
Assay type Sandwich ELISA
Standard curve range 13.72~10000 pg/ml
Sensitivity 5.76 pg/ml (50 μl)
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Rat
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.0495 | 0.0465 | 0.048 | |
| 13.72 | 0.052 | 0.049 | 0.0505 | 0.0025 |
| 41.15 | 0.0527 | 0.0553 | 0.054 | 0.006 |
| 123.46 | 0.073 | 0.0699 | 0.0715 | 0.0235 |
| 370.37 | 0.1478 | 0.1542 | 0.151 | 0.103 |
| 1111.11 | 0.5098 | 0.5151 | 0.5125 | 0.4645 |
| 3333.33 | 1.837 | 1.845 | 1.841 | 1.793 |
| 10000 | 3.6243 | 3.6317 | 3.628 | 3.58 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 138.5 | 903.8 | 2966 | 205.4 | 1327.9 | 4048.8 |
| Standard Deviation | 9.5 | 53.04 | 101.7 | 6.9 | 70.5 | 267 |
| Coefficient of Variation(%) | 6.9 | 5.9 | 3.4 | 3.3 | 5.3 | 6.6 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-18.96 | 13 | 9.23 |
Sensitivity
5.76 pg/ml (50 μl)
Standard curve range
13.72~10000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of rat IL-10 into health rat serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 73% to 115% with an overall mean recovery of 94%.
Specifications
Product Type
ELISA Kit
Product Name
Rat IL-10 enzyme-linked immunoassay kit (ER0005)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
CITATIONS
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Chen Jingyu,et al
Ginsenoside compound K plays an anti-inflammatory effect without inducing glucose metabolism disorder in adjuvant-induced arthritis rats
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Elisa
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Rat
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Citation
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