Presenilin 2 / AD5 Recombinant Rabbit Monoclonal Antibody [PSH11-18] (HA723300)
Catalog# HA723300
Rabbit Monoclonal to Presenilin 2 / AD5
Tested for
-
WB
-
IF-Cell
-
FC
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Presenilin 2 / AD5 on different lysates with Rabbit anti-Presenilin 2 / AD5 antibody (HA723300) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (no heat)
Lane 2: SH-SY5Y cell lysate (no heat)
Lane 3: RAW264.7 cell lysate (no heat)
Lane 4: Neuro-2a cell lysate (no heat)
Lane 5: PC-12 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 23 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 3: 1 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723300) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (no heat)
Lane 2: SH-SY5Y cell lysate (no heat)
Lane 3: RAW264.7 cell lysate (no heat)
Lane 4: Neuro-2a cell lysate (no heat)
Lane 5: PC-12 cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 23 kDa
Exposure time: Lane 1-4: 3 minutes; Lane 3: 1 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723300) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.