PPAR Rabbit Polyclonal Antibody (HA500569)
Catalog# HA500569
Rabbit Polyclonal to PPAR
Tested for
-
WB
Reacts with
-
Mouse
-
Rat
Western blot analysis of PPAR on different lysates with Rabbit anti-PPAR antibody (HA500569) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 42/52 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500569) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse skeletal muscle tissue lysate
Lane 3: Rat liver tissue lysate
Lane 4: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 42/52 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500569) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.