Podoplanin Rabbit Polyclonal Antibody (HA500364)
Catalog# HA500364
Rabbit Polyclonal to Podoplanin
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Western blot analysis of Podoplanin on different lysates with Rabbit anti-Podoplanin antibody (HA500364) at 1/1,000 dilution.
Lane 1: Human lung tissue lysate, 20 µg/Lane.
Lane 2: Human placenta tissue lysate, 20 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 37 kDa
Exposure time: 67 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500364) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Human lung tissue lysate, 20 µg/Lane.
Lane 2: Human placenta tissue lysate, 20 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 37 kDa
Exposure time: 67 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500364) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.