Phospho-Syk (Y526) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-78] (HA751911)
Catalog# HA751911
Rabbit Monoclonal to Phospho-Syk (Y526)
Tested for
-
WB
-
IP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Syk (Y526) on different lysates with Rabbit anti-Phospho-Syk (Y526) antibody (HA751911) at 1/5,000 dilution.
Lane 1: Ramos (Human Burkitt's lymphoma cells) cell lysate
Lane 2: Ramos treated with 12ug/mL human IgM for 10min cell lysate
Lane 3: Ramos treated with 12ug/mL human IgM for 10min cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Exposure time: 42 seconds; ECL: K1801
Total Syk protein was detected with another antibody (abcam, ab40781)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751911, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 72 kDa
Observed band size: 72 kDa
Western blot analysis of Phospho-Syk (Y526) on different lysates with Rabbit anti-Phospho-Syk (Y526) antibody (HA751911) at 1/5,000 dilution.
Lane 1: Ramos (Human Burkitt's lymphoma cells) cell lysate
Lane 2: Ramos treated with 12ug/mL human IgM for 10min cell lysate
Lane 3: Ramos treated with 12ug/mL human IgM for 10min cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Exposure time: 42 seconds; ECL: K1801
Total Syk protein was detected with another antibody (abcam, ab40781)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751911, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 72 kDa
Observed band size: 72 kDa