Phospho-STAT1 (Y701) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH04-02] (HA750925)
Catalog# HA750925
Rabbit Monoclonal to Phospho-STAT1 (Y701)
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-STAT1 (Y701) on different lysates with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA750925) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 91/84 kDa (STAT1α/β)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750925) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-STAT1 (Y701) on different lysates with Rabbit anti-Phospho-STAT1 (Y701) antibody (HA750925) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL IFNγ for 30 minutes cell lysate
Lane 3: HeLa cell lysate
Lane 4: HeLa treated with 50ng/mL IFNα1 for 30 minutes cell lysate
Lane 5: A431 cell lysate
Lane 6: A431 treated with 100ng/mL EGF for 30 minutes cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 91/84 kDa (STAT1α/β)
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750925) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.