Skip to content

Contact

(857) 353-6600

orders@huabio.com

Phospho-SAPK/JNK (T183/Y185) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-76] (HA751954)

Price: Regular price $649.00

Unit price per 

Product size

Bulk Order Quote   |   Customization Request

Catalog# HA751954

Rabbit Monoclonal to Phospho-SAPK/JNK (T183/Y185)

Tested for

  • WB

  • IHC-P

  • IP

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

Safety datasheet

Select your chosen country/region

COA DOWNLOAD

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Phospho-SAPK/JNK (T183/Y185) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-76] (HA751954)

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding thr183/tyr185 of human sapk/jnk aa 161-204.

Host

Rabbit

Modification

Phospho

Modification Site

T183/Y185

Positive Control

HeLa treated with 25μg/mL anisomycin for 30 minutes cell lysate, NIH/3T3 treated with 25μg/mL anisomycin for 30 minutes cell lysate, C6 treated with 25μg/mL anisomycin for 30 minutes cell lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

PSH24-76

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IHC-P

  • 1:1,000

  • IP

  • 1-2μg/sample

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 48 kDa

Isotype

IgG

TARGET

UNIPROT #

P45983

PROTEIN NAME

Phospho-SAPK/JNK (T183/Y185)

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Synapse.

FUNCTION

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase. Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member. There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.

Close (esc)

Popup

Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Enter

Search

Main menu

Your cart is currently empty.
Shop now