Phospho-MLKL (S358) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-31] (HA751701)
Catalog# HA751701
Rabbit Monoclonal to Phospho-MLKL (S358)
Tested for
-
WB
-
Dot Blot
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-MLKL (S358) on different lysates with Rabbit anti-Phospho-MLKL (S358) antibody (HA751701) at 1/5,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: HT-29 treated with 20μM Z-VAD for 7.5 hours add 20ng/mL hTNF alpha and 100nM SM-164 for last 7 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751701) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-MLKL (S358) on different lysates with Rabbit anti-Phospho-MLKL (S358) antibody (HA751701) at 1/5,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: HT-29 treated with 20μM Z-VAD for 7.5 hours add 20ng/mL hTNF alpha and 100nM SM-164 for last 7 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minute 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751701) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.