Phospho-Hormone sensitive lipase (S660) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH10-28] (HA751345)
Catalog# HA751345
Rabbit Monoclonal to Phospho-Hormone sensitive lipase (S660)
Tested for
-
WB
Reacts with
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Mouse
-
Rat
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Hormone sensitive lipase (S660) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S660) antibody (HA751345) at 1/2,000 dilution.
Lane 1: Mouse white adipose tissue lysate
Lane 2: Rat white adipose tissue lysate
Lane 3: Mouse white adipose tissue lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 40 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 84 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751345) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Hormone sensitive lipase (S660) on different lysates with Rabbit anti-Phospho-Hormone sensitive lipase (S660) antibody (HA751345) at 1/2,000 dilution.
Lane 1: Mouse white adipose tissue lysate
Lane 2: Rat white adipose tissue lysate
Lane 3: Mouse white adipose tissue lysate, the membrane treated with λpp for 1 hour
Lysates/proteins at 40 µg/Lane.
Predicted band size: 117 kDa
Observed band size: 84 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751345) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.