Phospho-GSK3 alpha (S21) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-11] (HA751265)
Catalog# HA751265
Rabbit Monoclonal to Phospho-GSK3 alpha (S21)
Tested for
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WB
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IHC-P
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Dot Blot
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA751265) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751265) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-GSK3 alpha (S21) on different lysates with Rabbit anti-Phospho-GSK3 alpha (S21) antibody (HA751265) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: A549 treated with 100nM Calyculin A for 30 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 200nM Calyculin A and 1μM Okadaic Acid for 1 hour cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751265) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.