Phospho-eIF4EBP1 (T70) Recombinant Rabbit Monoclonal Antibody [PSH09-91] (HA723222)
Catalog# HA723222
Rabbit Monoclonal to Phospho-eIF4EBP1 (T70)
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-eIF4EBP1 (T70) on different lysates with Rabbit anti-Phospho-eIF4EBP1 (T70) antibody (HA723222) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 treated with 20% FBS for 3 hours cell lysate
Lane 3: HEK-293 treated with 20% FBS for 3 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 13 kDa
Observed band size: 17/18 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-eIF4EBP1 (T70) on different lysates with Rabbit anti-Phospho-eIF4EBP1 (T70) antibody (HA723222) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HEK-293 treated with 20% FBS for 3 hours cell lysate
Lane 3: HEK-293 treated with 20% FBS for 3 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 13 kDa
Observed band size: 17/18 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723222) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.