Phospho-c-Fos (S32) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH21-99] (HA751815)
Catalog# HA751815
Rabbit Monoclonal to Phospho--c-Fos (S32)
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-c-Fos (S32) on different lysates with Rabbit anti-Phospho-c-Fos (S32) antibody (HA751815) at 1/5,000 dilution.
Lane 1: HeLa starved for 16 hours cell lysate
Lane 2: HeLa starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate
Lane 3: HeLa starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 55 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751815) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-c-Fos (S32) on different lysates with Rabbit anti-Phospho-c-Fos (S32) antibody (HA751815) at 1/5,000 dilution.
Lane 1: HeLa starved for 16 hours cell lysate
Lane 2: HeLa starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate
Lane 3: HeLa starved for 16 hours then treated with 200nM TPA for 4 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 41 kDa
Observed band size: 55 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751815) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.