Phospho-Axl (Y702) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH17-98] (HA751647)
Catalog# HA751647
Rabbit Monoclonal to Phospho-Axl (Y702)
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-Axl (Y702) on different lysates with Rabbit anti-Phospho-Axl (Y702) antibody (HA751647) at 1/5,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate
Lane 3: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751647) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-Axl (Y702) on different lysates with Rabbit anti-Phospho-Axl (Y702) antibody (HA751647) at 1/5,000 dilution.
Lane 1: NCI-H1299 cell lysate
Lane 2: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate
Lane 3: NCI-H1299 treated with 100ng/mL Gas6 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 98 kDa
Observed band size: 140 kDa
Exposure time: 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751647) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.