Phospho-ATR (T1989) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PS01-18] (HA750524)
Catalog# HA750524
Rabbit Monoclonal to Phospho-ATR (T1989)
Tested for
-
WB
-
IF-Cell
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with Hydroxyurea cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750524) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-ATR (T1989) on different lysates with Rabbit anti-Phospho-ATR (T1989) antibody (HA750524) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with Hydroxyurea cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750524) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.