Phospho-AKT (S473) Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH04-44] (HA750953)
Catalog# HA750953
Rabbit Monoclonal to Phospho-AKT (S473)
Tested for
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WB
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of Phospho-AKT (S473) on different lysates with Rabbit anti-Phospho-AKT (S473) antibody (HA750953) at 1/2,000 dilution and pan AKT antibody (HA721870) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 9: C6 cell lysate
Lane 10: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 11: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA for 1 hour at room temperature. The primary antibody (HA750953) at 1/2,000 dilution and pan AKT antibody (HA721870) at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Phospho-AKT (S473) on different lysates with Rabbit anti-Phospho-AKT (S473) antibody (HA750953) at 1/2,000 dilution and pan AKT antibody (HA721870) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 5: HEK-293 cell lysate
Lane 6: HEK-293 treated with 50μM LY294002 for 6 hours cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: NIH/3T3 treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 9: C6 cell lysate
Lane 10: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate
Lane 11: MCF7 treated with 50ng/mL Calyculin A for 45 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA for 1 hour at room temperature. The primary antibody (HA750953) at 1/2,000 dilution and pan AKT antibody (HA721870) at 1/2,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.