PFKM Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JU53-31] (HA751831)
Catalog# HA751831
Rabbit Monoclonal to PFKM
Tested for
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PFKM on different lysates with Rabbit anti-PFKM antibody (HA751831) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Ramos cell lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Mouse skeletal muscle tissue lysate
Lane 5: Rat heart tissue lysate
Lane 6: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751831) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: Ramos cell lysate
Lane 3: Mouse heart tissue lysate
Lane 4: Mouse skeletal muscle tissue lysate
Lane 5: Rat heart tissue lysate
Lane 6: Rat skeletal muscle tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 75 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751831) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.