Peroxiredoxin 4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-40] (HA750874)
Catalog# HA750874
Rabbit Monoclonal to Peroxiredoxin 4
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Peroxiredoxin 4 on different lysates with Rabbit anti-Peroxiredoxin 4 antibody (HA750874) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: LNCaP cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 27 kDa
Exposure time: 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750874) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: LNCaP cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 27 kDa
Exposure time: 50 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750874) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.