Periostin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-35] (HA751936)
Catalog# HA751936
Rabbit Monoclonal to Periostin
Tested for
-
WB
-
IHC-Fr
-
IHC-P
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (HA751936) at 1/5,000 dilution.
Lane 1: Mouse skin tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lane 4: Mouse stomach tissue lysate
Low expression: Mouse spleen (PMID: 18086302), Mouse skeletal muscle (PMID: 15731169)
Lysates/proteins at 30 µg/Lane.
Exposure time: 43 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751936, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 93 kDa
Observed band size: 85 kDa
Western blot analysis of Periostin on different lysates with Rabbit anti-Periostin antibody (HA751936) at 1/5,000 dilution.
Lane 1: Mouse skin tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse skeletal muscle tissue lysate
Lane 4: Mouse stomach tissue lysate
Low expression: Mouse spleen (PMID: 18086302), Mouse skeletal muscle (PMID: 15731169)
Lysates/proteins at 30 µg/Lane.
Exposure time: 43 seconds; ECL: K1802
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751936, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 93 kDa
Observed band size: 85 kDa