PCK1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-82] (HA751915)
Catalog# HA751915
Rabbit Monoclonal to PCK1
Tested for
-
WB
Reacts with
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of PCK1 on different lysates with Rabbit anti-PCK1 antibody (HA751915) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse kidney tissue lysate
Lane 4: Mouse brown adipose tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat spleen tissue lysate
Lane 7: Rat kidney tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 40 seconds; ECL: K1801
Low expression: spleen (PMID: 36918564)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751915, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 69 kDa
Observed band size: 69 kDa
Western blot analysis of PCK1 on different lysates with Rabbit anti-PCK1 antibody (HA751915) at 1/5,000 dilution.
Lane 1: Mouse liver tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse kidney tissue lysate
Lane 4: Mouse brown adipose tissue lysate
Lane 5: Rat liver tissue lysate
Lane 6: Rat spleen tissue lysate
Lane 7: Rat kidney tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 40 seconds; ECL: K1801
Low expression: spleen (PMID: 36918564)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751915, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 69 kDa
Observed band size: 69 kDa