PAI1 Recombinant Rabbit Monoclonal Antibody [PSH24-33] (HA724440)
Catalog# HA724440
Rabbit Monoclonal to PAI1
Tested for
-
WB
-
IP
Reacts with
-
Human
-
Mouse
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE),Cell treatment (CT)
Western blot analysis of PAI1 on different lysates with Rabbit anti-PAI1 antibody (HA724440) at 1/5,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cells) cell lysate
Lane 2: A549 treated with 5ng/ml hTGFβ1 for 48 hours and 300ng/ml BFA for 16 hours cell lysate
Lane 3: EA.hy926 (Human umbilical vein fusion cells) cell lysate
Lane 4: HCT 116 (Human colon cancer cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
HCT 116 is a negative control (PMID: 32344898).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted MW: 45 kDa
Observed MW: 45 kDa
Western blot analysis of PAI1 on different lysates with Rabbit anti-PAI1 antibody (HA724440) at 1/5,000 dilution.
Lane 1: A549 (Human lung adenocarcinoma cells) cell lysate
Lane 2: A549 treated with 5ng/ml hTGFβ1 for 48 hours and 300ng/ml BFA for 16 hours cell lysate
Lane 3: EA.hy926 (Human umbilical vein fusion cells) cell lysate
Lane 4: HCT 116 (Human colon cancer cells) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
HCT 116 is a negative control (PMID: 32344898).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724440, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted MW: 45 kDa
Observed MW: 45 kDa