p27 KIP 1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [SU37-04] (HA750157)

UNIPROT #

P46527

PROTEIN NAME

p27 KIP 1

SYNONYMS

AA408329 antibody;AI843786 antibody;Cdki1b antibody;CDKN 1B antibody;CDKN 4 antibody;CDKN1B antibody;CDKN4 antibody;CDN1B_HUMAN antibody;Cyclin Dependent Kinase Inhibitor 1B antibody;Cyclin dependent kinase inhibitor p27 antibody;Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody;Cyclin-dependent kinase inhibitor 1B antibody;Cyclin-dependent kinase inhibitor p27 antibody;Cyclin-dependent kinase inhibitor p27 Kip1 antibody;KIP 1 antibody;KIP1 antibody;MEN1B antibody;MEN4 antibody;OTTHUMP00000195098 antibody;OTTHUMP00000195099 antibody;p27 antibody;p27 Kip1 antibody;P27-like cyclin-dependent kinase inhibitor antibody;p27Kip1 antibody

SEQUENCE SIMILARITIES

Belongs to the CDI family.

TISSUE SPECIFICITY

Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK1 and CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.; Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.; Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation (By similarity).

SUBCELLULAR LOCATION

Nucleus, Cytoplasm, Endosome.

FUNCTION

Cyclin-dependent kinase inhibitor 1B (p27Kip1) is an enzyme inhibitor that in humans is encoded by the CDKN1B gene. It encodes a protein which belongs to the Cip/Kip family of cyclin dependent kinase (Cdk) inhibitor proteins. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. It is often referred to as a cell cycle inhibitor protein because its major function is to stop or slow down the cell division cycle.