P-Selectin / CD62P Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-03] (HA751693)
Catalog# HA751693
Rabbit Monoclonal to P-Selectin / CD62P
Tested for
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WB
-
IHC-P
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FC
-
IHC-Fr
-
IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of P-Selectin / CD62P on different lysates with Rabbit anti-P-Selectin / CD62P antibody (HA751693) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (negative) (20 µg/Lane)
Lane 2: HEL cell lysate (20 µg/Lane)
Lane 3: Mouse placenta tissue lysate (40 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)
Lane 5: Rat placenta tissue lysate (40 µg/Lane)
Lane 6: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 130-150 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751693) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of P-Selectin / CD62P on different lysates with Rabbit anti-P-Selectin / CD62P antibody (HA751693) at 1/2,000 dilution.
Lane 1: HeLa cell lysate (negative) (20 µg/Lane)
Lane 2: HEL cell lysate (20 µg/Lane)
Lane 3: Mouse placenta tissue lysate (40 µg/Lane)
Lane 4: Mouse lung tissue lysate (40 µg/Lane)
Lane 5: Rat placenta tissue lysate (40 µg/Lane)
Lane 6: Rat lung tissue lysate (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 130-150 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751693) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.