NUPR1 Rabbit Polyclonal Antibody (HA500637)
Catalog# HA500637
Rabbit Polyclonal to NUPR1
Tested for
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WB
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IF-Cell
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FC(Intra)
Reacts with
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Human
Western blot analysis of NUPR1 on different lysates with Rabbit anti-NUPR1 antibody (HA500637) at 1/5,000 dilution.
Lane 1: 786-0 (Human renal clear cell adenocarcinoma cells) cell lysate
Lane 2: U-87 MG (Human glioblastoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500637, 1/5,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 9 kDa
Observed band size: 14 kDa
Lane 1: 786-0 (Human renal clear cell adenocarcinoma cells) cell lysate
Lane 2: U-87 MG (Human glioblastoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500637, 1/5,000 in 5% BSA, overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 9 kDa
Observed band size: 14 kDa