NR4A3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-01] (HA751923)
Catalog# HA751923
Rabbit Monoclonal to NR4A3
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of NR4A3 on different lysates with Rabbit anti-NR4A3 antibody (HA751923) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 2: THP-1 treated with 20ng/ml TPA for 24 hours and 20ng/ml IL-4 for 48 hours cell lysate
Lane 3: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 4: THP-1 treated with 50ng/ml TPA for 72 hours and 1μg/ml LPS for 24 hours cell lysate
Lane 5: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 6: THP-1 treated with 80nM TPA for 16 hours and 20ng/ml hIFN-γ for 8 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751923, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 68 kDa
Observed band size: 68 kDa
Western blot analysis of NR4A3 on different lysates with Rabbit anti-NR4A3 antibody (HA751923) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 2: THP-1 treated with 20ng/ml TPA for 24 hours and 20ng/ml IL-4 for 48 hours cell lysate
Lane 3: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 4: THP-1 treated with 50ng/ml TPA for 72 hours and 1μg/ml LPS for 24 hours cell lysate
Lane 5: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 6: THP-1 treated with 80nM TPA for 16 hours and 20ng/ml hIFN-γ for 8 hours cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751923, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 68 kDa
Observed band size: 68 kDa