NQO1 Recombinant Mouse Monoclonal Antibody - BSA and Azide free [13A1-R] (HA610087)
Catalog# HA610087
Mouse Monoclonal to NQO1
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
☑ Relative expression (RE)
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (HA610087) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NQO1 on different lysates with Mouse anti-NQO1 antibody (HA610087) at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: SiHa cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: HeLa cell lysate
Lane 6: Saos-2 cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.