NPTX2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH21-53] (HA751800)
Catalog# HA751800
Rabbit Monoclonal to NPTX2
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of NPTX2 on different lysates with Rabbit anti-NPTX2 antibody (HA751800) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A-172 cell lysate (negative)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse kidney tissue lysate (negative)
Lane 5: Rat brain tissue lysate
Lane 6: Rat kidney tissue lysate (negative)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 55 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751800) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of NPTX2 on different lysates with Rabbit anti-NPTX2 antibody (HA751800) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A-172 cell lysate (negative)
Lane 3: Mouse brain tissue lysate
Lane 4: Mouse kidney tissue lysate (negative)
Lane 5: Rat brain tissue lysate
Lane 6: Rat kidney tissue lysate (negative)
Lysates/proteins at 15 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 55 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751800) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.