NKX3.1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JE36-27] (HA751825)
Catalog# HA751825
Rabbit Monoclonal to NKX3.1
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NKX3.1 on different lysates with Rabbit anti-NKX3.1 antibody (HA751825) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: RWPE-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751825) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: RWPE-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 26 kDa
Observed band size: 30 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751825) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.