NKG2D Rabbit Polyclonal Antibody (HA500626)
Catalog# HA500626
Rabbit Polyclonal to NKG2D
Tested for
-
WB
Reacts with
-
Human
Western blot analysis of NKG2D on different lysates with Rabbit anti-NKG2D antibody (HA500626) at 1/5,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 34 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500626) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate
Lane 2: A549 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 24 kDa
Observed band size: 34 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500626) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.